collagenase type iv (Thermo Fisher)

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Thermo Fisher collagenase type iv
Collagenase Type Iv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+type+iv/Collagenase/pm42014405-308-19-22
Average 99 stars, based on 1 article reviews

collagenase type iv - by Bioz Stars, 2026-09

99/100 stars

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Incubation:

Article Title: HFTMSCs alleviate uterine fibrosis and restore fertility in intrauterine adhesion models.
Article Snippet: .. Sequential enzymatic digestion was then performed, tissues were first incubated with 1 mg/mL collagenase type IV (17104019, Thermo Fisher, USA) at 37°C for 70 minutes under continuous agitation, the digested material was subjected to centrifugation at 1,000 revolutions per minute for 10 minutes at ambient temperature to collect the fragmented components. .. The supernatant was carefully removed, and the remaining tissue was then treated with a 0.25% trypsin-EDTA solution (G4011, Servicebio, Wuhan, China) for 8 minutes under gentle agitation at 37°C.

Article Title: Exacerbated ATP transmission in the carotid body is linked to glomus cell expansion in spontaneously hypertensive rats
Article Snippet: figure legend Exacerbated ATP signalling in the carotid body (CB) of spontaneously hypertensive rats (SHRs) arises from expansion of the chemosensory glomus cell compartment and reduced extracellular ATP metabolism, © 2026 The Author(s).. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.. DOI: 10.1113/JP288931 This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial

Centrifugation:

Isolation:

Article Title: Integrated analysis of stemness-associated immune modulatory circuits in squamous cell carcinomas
Article Snippet: .. For isolation from grafted tumors, tumors were excised, minced, and enzymatically dissociated in RPMI-1640 (Gibco) containing 2 mg/mL collagenase type IV (Gibco) and 20 U/mL DNase I (Roche) for 60 min at 37°C with gentle agitation. ..

Gentle:

other:

Article Title: CK2 inhibition suppresses glial inflammation in models of neuroinflammation and neurodegeneration.
Article Snippet: hiPSC colonies were cultured on Cultrex RGF BME (R&D Systems; 3433-005-01)-coated, 6-well tissue culture-treated plates using mTeSR Plus (STEMCELL Technologies, 85850) and were maintained in 5% CO2 humidified incubators at 37 °C.

Knock-Out:

Article Title: A scalable human-zebrafish xenotransplantation model reveals gastrosome-mediated processing of dying neurons by human microglia.
Article Snippet: .. Human iPSC colonies were detached before reaching confluency with collagenase Type IV (Gibco) and transferred to an Ultra-Low attachment 10-cm plate (Corning Costar) containing 10 ml hPSC medium consisting of DMEM:F12 (Invitrogen), 20% Knockout Serum Replacement (Gibco), 1× Non-essential Amino Acids (Invitrogen), 1× 2-mercaptoethanol (Gibco), 1× GlutaMAX (Invitrogen), 10 ng ml–1 FGF-2 (Peprotech) and ROCK inhibitor Y27632 (10 μM). .. Twenty-four hours later, the medium was replaced with induction medium containing hPSC media without FGF-2, 2 μM dorsomorphin (Tocris) and 2 μM A-083 (Tocris).

Red Blood Cell Lysis:

Article Title: Immunopeptidome profiling in pulmonary fibrosis provides a platform for identifying therapeutic targets.
Article Snippet: .. For preparation of lung samples, lungs were minced and digested with 1 mg ml−1 collagenase type I (Gibco) + 0.5 mg ml−1 collagenase type IV (Gibco) in RPMI 1640 at 37 °C and 200 rpm for 45 min, filtered through a 70-μm strainer and washed with PBS, and red blood cell lysis was achieved with red blood cell lysis buffer (154 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA 2Na (pH 7.4)). ..

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